Ethidium Bromide Solution pour fluorescence - Solution à 1% dans l'eau
3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide
|
Référence : 8231995
Unité de vente : 250 ml
|
|
Application : Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids.1,2,3 It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA.4 Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.5
Biochem/physiol Actions : Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
Reconstitution : For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.
Classe danger : 6.1 / UN2810 - Groupe d'emballage : III.
Poids |
2 Kg |
Volume |
0,025 m³ (25000 cm³) |
Largeur |
0,25 m (25 cm) |
Longueur |
0,25 m (25 cm) |
Hauteur |
0,4 m (40 cm) |
Les dimensions, le poids et le volume sont bruts (emballage inclus)