BBL™ Phytone™ Yeast Extract Agar

Intended Use
Phytone Yeast Extract Agar is used for the selective isolation of dermatophytes, particularly Trichophyton verrucosum, and other pathogenic fungi from routine clinical specimens.

Summary and Explanation
Carmichael and Kraus modified the classical formula of Sabouraud medium in order to selectively recover Trichophyton verrucosum, one of the species associated with ringworm, from clinical specimens.

Phytone Yeast Extract Agar is used in Petri dishes for early detection of dermatophytes. Skin scrapings or hairs are rubbed over the surface of the agar. Blood agar plates should be inoculated in parallel to permit isolation of pyogenic Cocci which may also be present. The medium is of value for increasing the yield of isolation of ringworm organisms and for early identification, especially of T. verrucosum.

Principles of the Procedure
The incorporation of the antimicrobial agents streptomycin and chloramphenicol in the medium inhibits growth of bacterial species and promotes detection of pathogenic fungi.

Formula
BBL™ Phytone™ Yeast Extract Agar
Approximate Formula* Per Liter
Papaic Digest of Soybean Meal : 10.0 g
Yeast Extract : 5.0 g
Dextrose : 40.0 g
Streptomycin : 0.03 g
Chloramphenicol : 0.05 g
Agar : 17.0 g
*Adjusted and/or supplemented as required to meet performance criteria.

Procedure
Inoculate skin scrapings, hair or other materials directly on the agar surface of Petri plates. Incubate plates in an aerobic atmosphere at 25-30°C or at 30-37°C if T. verrucosum is suspected.

Expected Results
After the plates have been incubated for 2-3 days, examine them directly under the microscope. If microcolonies are observed, they should be transferred to fresh plates before the original plates become overgrown.

 

Poids	0,81 Kg   (810 g)
Volume	0,0094 m³   (9400 cm³)
Largeur	0,15 m   (15 cm)
Longueur	0,25 m   (25 cm)
Hauteur	0,25 m   (25 cm)

Les dimensions, le poids et le volume sont bruts (emballage inclus)