Difco™ Sabouraud Dextrose Agar

Intended Use
Sabouraud Dextrose Agar is used in qualitative procedures for cultivation of pathogenic and nonpathogenic fungi, particularly dermatophytes. The medium is rendered more selective for fungi by the addition of antimicrobics. Sabouraud Dextrose Broth and
Sabouraud Maltose Agar and Broth are also used for culturing yeasts, molds and aciduric microorganisms.
Fluid Sabouraud Medium is used for cultivating yeasts, molds and aciduric microorganisms and for detecting yeasts and molds in normally sterile materials.
Sterile Pack RODAC™ environmental sampling plates, containing Sabouraud Dextrose Agar with Lecithin and Polysorbate 80, are used for the detection and enumeration of microorganisms present on surfaces of sanitary importance. Sterile Pack plates are particularly  useful for monitoring surfaces in clean rooms and other environmentally-controlled areas and are also recommended for use in air sampling equipment, such as the Surface Air System.
Sterile Pack Finger Dab™ Isolator plates are intended for sampling gloved hands.
Sabouraud Dextrose Agar and Sabouraud Dextrose Broth meet United States Pharmacopeia (USP), European Pharmacopoeia (EP) and Japanese Pharmacopoeia (JP)1-3 performance specifications, where applicable.

Identity Specifications
Difco™ Sabouraud Dextrose Agar
Dehydrated Appearance: Light beige, free-flowing, homogeneous.
Solution: 6.5% solution, soluble in purified water upon boiling. Solution is light to medium amber, very slightly to slightly opalescent.
Prepared Appearance: Light to medium amber, slightly opalescent.
Reaction of 6.5%
Solution at 25°C: pH 5.6 ± 0.2

Cultural Response
Difco™ Sabouraud Dextrose Agar
Prepare the medium per label directions. Inoculate and incubate at 30± 2°C for 18-48 hours, or up to 7 days for Trichophyton. Incubate (*) cultures at 20-25°C for up to 5 days. Incubate (**) culture at 30-35°C for 48 hours.

ORGANISM / ATCC™ / INOCULUM CFU / RECOVERY
Saccharomyces cerevisiae / 9763 / 100-300 / Good
Trichophyton mentagrophytes / 9533 / Undiluted / Good
Aspergillus brasiliensis (niger)* / 16404 / <100 / Growth
Candida albicans* / 10231 / <100 / Growth
Candida albicans** / 10231 / <100 / Growth

Formulae
Difco™ Sabouraud Dextrose Agar
Approximate Formula* Per Liter
Peptic Digest of Animal Tissue : 5.0 g
Pancreatic Digest of Casein : 5.0 g
Dextrose : 40.0 g
Agar : 15.0 g
*Adjusted and/or supplemented as required to meet performance criteria.