Gélose sélective pour isolement des coliformes dans les eaux et principalement dans l'industrie laitière.

La gélose Endo (Endo agar) est un milieu de culture différentiel légèrement sélectif pour la détection de coliformes et autres microorganismes entériques.

La sélectivité de la gélose Endo est due à la combinaison du sulfite de sodium et de la fuchsine basique, qui entraîne la suppression partielle des micro-organismes gram positifs. Il est classé comme légèrement sélectif puisque d’autres milieux contiennent des inhibiteurs plus puissants pour les micro-organimes gram positifs.

Les coliformes fermentent le lactose, produisent des colonies rose foncé à rose-rouge présentant un aspect brillant et irisé vert métallisé avec coloration identique du milieu. Les colonies de micro-organismes qui ne fermentent pas le lactose, sont incolores voir rose pâle et s’estompent sur le fond rose du milieu.

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Intended Use
Endo Agar is a differential and slightly selective culture medium for the detection of coliform and other enteric microorganisms.

Summary and Explanation
The majority of the enteric plating media developed in the early years of the 20th century utilized either mixtures of bile salts or individual salts as selective agents to achieve inhibition of gram-positive species. In 1904, Endo reported the development of a culture medium for the differentiation of lactose fermenters from the nonfermenters in which no bile salts were used. Inhibition of gram-positive microorganisms was achieved by the sodium sulfite and basic fuchsin contained in the formulation. Endo’s Fuchsin Sulphite Infusion Agar was the original name for this medium, which is known today as Endo Agar. It was developed initially in order to facilitate the isolation and identification of the typhoid bacillus. The original formula has been modified extensively since its introduction. The meat infusions have been replaced by a peptic digest of animal tissue. The dye composition and concentration also have been adjusted.

Over the years, Endo Agar has been an important medium in the microbiological examination of potable water and wastewater, dairy products and foods; however, the current compendia of standard methods for the examination of these materials recommend alternative media formulations.

Principles of the Procedure
The selectivity of Endo Agar is due to the sodium sulfite/basic fuchsin combination, which results in the suppression of gram-positive microorganisms. It is classified as only slightly selective since other media contain more potent inhibitors of the gram-positive microorganisms. Coliforms ferment the lactose, produce pink to rose-red colonies and similar coloration of the medium. The colonies of organisms that do not ferment lactose are colorless to faint against the pink background of the medium.

Poids	0,72 Kg   (720 g)
Volume	0,0056 m³   (5600 cm³)
Largeur	0,15 m   (15 cm)
Longueur	0,15 m   (15 cm)
Hauteur	0,25 m   (25 cm)

Les dimensions, le poids et le volume sont bruts (emballage inclus)