General description
1-Fluoro-2,4-dinitrobenzene (DNFB) is a reagent that modifies the N-terminal amino acids of proteins and peptides. DNFB is also commonly known as Sanger′s Reagent, named after Frederick Sanger, who reported in a classic 1945 paper the use of DNFB for derivatization of proteins and peptides specifically at their N-termini.†

Application
1-Fluoro-2,4-dinitrobenzene has been used to induce severe contact hypersensitivity in mice and to monitor gene expression by in vivo imaging method, and to induce chronic itch as a model for analysing itch-scratching behavior.

Biochem/physiol Actions
1-Fluoro-2,4-dinitrobenzene is a contact-sensitizing hapten, commonly used in experimental studies on contact hypersensitivity, inducing itch model, and atopic dermatitis. Mast cell activation is a key step in the DNFB induced sensitivity. DNFB covalently binds to the N-terminal amino acid of the protein and aids in Sanger sequencing.

Refractive index : n_20/D = 1.569 (lit.)
bp : 178 °C/25 mmHg (lit.)
mp : 25-27 °C (lit.)
Density : 1.482 g/mL at 25°C (lit.)

Poids	0,45 Kg   (450 g)
Volume	0,0015 m³   (1500 cm³)
Largeur	0,1 m   (10 cm)
Longueur	0,1 m   (10 cm)
Hauteur	0,1 m   (10 cm)

Les dimensions, le poids et le volume sont bruts (emballage inclus)