Intended Use
Bacto Yeast Extract, Yeast Extract, UF (ultra-filtered), Yeast Extract, LD, Bacto Yeast Extract, Technical and Yeast Extract are used in preparing microbiological culture media.
Summary and Explanation
Bacto Yeast Extract, Yeast Extract, UF, Yeast Extract, LD, Bacto Yeast Extract, Technical and Yeast Extract are concentrates of the water-soluble portion of Saccharomyces cerevisiae cells that have been autolyzed. The autolysis is carefully controlled to preserve the naturally occurring B-complex vitamins. Yeast extract is considered a non-animal product and is used extensively for many non-animal formulations for bacterial, fungal, mammalian and insect cell culture.
Bacto Yeast Extract has been considered one of the most complete and versatile of the fermentation bionutrients available. It has been a valuable ingredient for the microbiological assay of vitamins. Yeast extract is also of value in the assay of antibiotics. B factor, a growth substance necessary for the production of rifampin in a Nocardia sp., can be isolated from yeast extract. Yeast Extract, UF is ultra-filtered and specifically designed for tissue culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it is an ideal substitute
for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g. Yeast Extract, LD was created to eliminate the problem of dust inhalation when handling large quantities of yeast extract. Yeast Extract, Yeast Extract, UF and Yeast Extract, LD are processed from the same culture of Saccharomyces. Bacto Yeast Extract, Technical and Yeast Extract were developed to provide products priced for the biotechnology/pharmaceutical market with acceptable clarity and growth promoting characteristics.
Media formulations containing yeast extract are specified in standard methods for various applications.
Principles of the Procedure
Bacto Yeast Extract, Yeast Extract, UF, Yeast Extract, LD, Bacto Yeast Extract, Technical and Yeast Extract are prepared by growing baker’s yeast, Saccharomyces sp., in a carbohydrate-rich plant medium. The yeast is harvested, washed and resuspended in water, where it undergoes autolysis, or self-digestion. Yeast extract is the total soluble portion of this autolytic action. The autolytic activity is stopped by a heating step. The resulting yeast extract is then filtered to produce a clear product and subsequently made into a powder by a spray-drying process. Bacto Yeast Extract, Yeast Extract, UF, Yeast Extract, LD, Bacto Yeast Extract, Technical and Yeast Extract provide vitamins, nitrogen, amino acids and carbon in microbiological culture media.
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