Difco™ TAT Broth Base • TAT Broth

Intended Use
TAT Broth Base with added polysorbate 20 and TAT Broth (complete) are used for cultivating microorganisms from highly viscous or gelatinous materials.

Summary and Explanation
TAT (Tryptone-Azolectin-Tween™*) Broth Base with the addition of polysorbate 20 is recommended for testing for the presence of microorganisms in viscous materials, such as salves or ointments. It is especially adapted to the testing of cosmetics. Cosmetics and pharmaceutical products are subject to contamination during manufacturing and use by consumers.1
Preservatives are used in aqueous products to make them self-sterilizing for vegetative bacteria, yeasts and molds, and bacteriostatic or bactericidal for spores.1 TAT Broth is the medium, including polysorbate 20, provided in bottles.

User Quality Control
Identity Specifications
Difco™ TAT Broth Base
Dehydrated Appearance: Beige, free-flowing, homogeneous.
Solution: 2.5% solution with 4% polysorbate 20. Soluble when heated to 50-60°C ; let stand for 15-30 minutes with occasional agitation to dissolve prior to autoclaving. Solution is light amber, clear to very slightly opalescent, may have a very slight precipitate. Prepared Appearance: Light amber, clear to very slightly opalescent, with a very sight precipitate. Reaction of 2.5% Solution w/4% Polysorbate 20 at 25°C: pH 7.2 ± 0.2

Principles of the Procedure
Peptone provides the nitrogen, vitamins, amino acids and carbon in TAT Broth Base. Soy lecithin and polysorbate 20 neutralize preservatives in the cosmetics or pharmaceutical products, allowing bacteria to grow.

Formulae
Difco™ TAT Broth Base
Approximate Formula* Per 960 mL
Pancreatic Digest of Casein : 20.0 g
Soy Lecithin : 5.0 g