Difco™ Rappaport Vassiliadis Salmonella (RVS) Soy Broth

Intended Use
Rappaport Vassiliadis Salmonella (RVS) Soy Broth is used for selectively enriching Salmonella in food and environmental samples.
Meets United States Pharmacopeia (USP), European Pharmacopoeia (EP) and Japanese Pharmacopoeia (JP)1-3 performance specifications, where applicable.

Principles of the Procedure
RVS Soy Broth contains soy peptone as the carbon and nitrogen source for general growth requirements. Magnesium chloride raises the osmotic pressure in the medium. Sodium chloride maintains osmotic balance. Dipotassium phosphate and potassium dihydrogen phosphate are buffering agents. Malachite green is inhibitory to organisms other than salmonellae. The low pH of the medium, combined with the presence of malachite green and magnesium chloride, helps to select for the highly resistant Salmonella spp.

Formula
Difco™ RVS Soy Broth
Approximate Formula* Per Liter
Soy Peptone : 4.5 g
Magnesium Chloride (anhydrous) : 13.5 g
Sodium Chloride : 9.0 g
Dipotassium Phosphate : 0.03 g
Potassium Dihydrogen Phosphate : 1.45 g
Malachite Green : 36.0 mg
*Adjusted and/or supplemented as required to meet performance criteria.

NOTE: Formula is further adjusted from the USP formulation as follows. Since magnesium chloride hexahydrate contains too much water to be effectively used in the manufacture of dehydrated culture media, magnesium chloride anhydrous (without water) is substituted. The actual amount of magnesium chloride (minus water) is the same. However, the use of the anhydrous magnesium requires slight adjustments in the rest of the formulation.
None of these slight changes affect performance, as is indicated on the Certificate of Analysis, which shows that harmonized USP/EP/JP growth promotion criteria are met per requirements for the Microbiological Examination of Nonsterile Products.

User Quality Control
Identity Specifications
Difco™ RVS Soy Broth
Dehydrated Appearance: Pale green to green, free-flowing, homogeneous.
Solution: 2.85% solution, soluble in purified water upon gentle heating. Solution is blue, clear.
Prepared Appearance: Blue, clear.
Reaction of 2.85%
Solution at 25°C: pH 5.2 ± 0.2

Cultural Response
Difco™ RVS Soy Broth
Prepare the medium per label directions. Inoculate and incubate at 30-35°C for 24 hours. After incubation, subculture to XLD Agar plates and incubate at 30-35°C for 18-48 hours.

ORGANISM / ATCC™ / INOCULUM CFU / RECOVERY
Salmonella enterica subsp. enterica serotype Typhimurium / 14028 / <100 / Growth
Staphylococcus aureus / 6538 / >100 / No growth

Procedure
Refer to appropriate references for details on test methods using RVS Soy Broth. Inoculate tubes with the test sample and incubate as instructed in appropriate references.

Expected Results
Examine selective plates for typical Salmonella colonies. Confirm identification of isolates by biochemical and/or serological tests as directed in appropriate references.

Limitation of the Procedure
The combined inhibitory factors of this medium (malachite green, magnesium chloride, low pH) may inhibit certain Salmonella, such as S. Typhi and S. Paratyphi A. Isolation techniques should include a variety of enrichment broths and isolation media.

Poids	0,81 Kg   (810 g)
Volume	0,0094 m³   (9400 cm³)
Largeur	0,15 m   (15 cm)
Longueur	0,25 m   (25 cm)
Hauteur	0,25 m   (25 cm)

Les dimensions, le poids et le volume sont bruts (emballage inclus)