Analysis Note : The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Biochem/physiol Actions : Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

General description : Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Other Notes : View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

Reconstitution : When reconstituted with 1 ml H₂O, one vial will contain 10 mg bovine serum albumin, 0.15 M NaCl and 0.05 M sodium citrate, pH 6.5

Unit Definition : Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

Application : Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study that compared efficacy and safety of low-molecular-weight heparins (LMWHs) to other anticoagulants as venous thromboembolism prophylaxis in major orthopedic surgery.

Poids	1,8 Kg
Volume	0,0156 m³   (15600 cm³)
Largeur	0,25 m   (25 cm)
Longueur	0,25 m   (25 cm)
Hauteur	0,25 m   (25 cm)

Les dimensions, le poids et le volume sont bruts (emballage inclus)