Colonne analytique pour GFC (Gel Filtration Chromatography) - Gamme Phenomenex™ BioSep™

GFC is used for the analysis and/or characterization of proteins, peptides and other biomolecules; including antibodies, immunoglobulins, protein complexes, protein aggregates, and desalting.

BioSep GFC columns offer many important benefits to keep your research, method development/validation, and ongoing size exclusion separations SIMPLE:

High Performance:
Analytical and preparative BioSep columns offer high resolution, maximum efficiency, and exceptional peak asymmetry.

Easy Column Selection:
Simply choose the right phase based on the MW of your sample, recommended application, currently used GFC column, or contact us for assistance!

Higher Value Solution:
BioSep is a high quality gel filtration media that comes with an affordable price tag.

Method Development and Optimization Services:
Phenomenex Services offers method development and optimization support for new methods, as well as transferring your current methods from other GFC media to BioSep-SEC-S phases.

BioSep SEC-s2000 SEC/GFC phase has an exclusion range of:
    1,000 - 300,000 Da (Native)
    200 - 75,000 Da (0.5% SDS)
    500 - 100,000 Da (6M GnHCl)

 

Technical Data and Specifications

Resin Type : Silica
Particle Size (μm) : 5
Pore Size (Å) : 145
pH Range : 2.5 - 7.5
Maximum Backpressure (psi) : 1,500
Typical Backpressure (psi) : 800
Efficiency (minimum number theoretical plates L.300 x Ø.7.8 mm) : 30,000
Maximum Flow Rate : This is a function of pressure. Columns can withstand up to 1,500 psi, but avoid sudden pressure changes.
Column Hardware : Standard: 316 stainless steel column with stainless steel frits. Titanium frits available.
Maximum Temp.: 50˚C
Maximum Salt Conc.: 1 M
Denaturants : 0.5 % SDS, 6 M Guanidine HCI, or 8 M urea
Regeneration : After exposure to denaturants, wash with water overnight.
Max. Organic Modifier : Up to 100 % CH₃CN. Start with 100 % H₂O, linear gradient to 100 % CH₃CN over 50 min. Up to 90 % CH₃CN, 10 % DMSO or 500 mM b-mercaptoethanol.

Cleaning Procedure General protein removal : wash with 30 mL of 0.1 M NaH₂PO₄ , pH 3.0. Hydrophobic protein removal: use acetonitrile gradient. Strongly adsorbed proteins: wash with 30 mL of 0.5 % SDS or 6 M Guanidine thiocyanate or 10 % DMSO.

Storage Overnight storage : run mobile phase at 0.2 mL/minute. Prolonged storage : use 0.05 % NaN₃ in H₂O or 10 % methanol in H₂O.
Column Protection : Use of a SecurityGuard is recommended to prolong column lifetime.

Recommended Use
Ideal for biogeneric aggregate applications, peptide therapeutics, small proteins, PEGylated peptides, and PEGylated small proteins.

Poids	0,81 Kg   (810 g)
Volume	0,006 m³   (6000 cm³)
Largeur	0,15 m   (15 cm)
Longueur	0,4 m   (40 cm)
Hauteur	0,1 m   (10 cm)

Les dimensions, le poids et le volume sont bruts (emballage inclus)