Product Description
BD Bacto™ Peptone is an enzymatic digest of animal protein. This peptone was first introduced in 1914 and became the standard peptone for the preparation of bacteriological culture media. The nutritive value of BD Bacto™ Peptone is largely dependent on the amino acid content, which supplies essential nitrogen. BD Bacto™ Peptone contains only a negligible quantity of proteoses and more complex constituents.
Potential Applications
BD Bacto™ Peptone is used as an organic nitrogen source in microbiological culture media for cultivation of a variety of bacteria and fungi. For example, Iwanaga et al(1) utilized BD Bacto™ Peptone for production of cholera toxin by Vibrio cholerae O1 El Tor. Benkerroum et al(2) reported using BD Bacto™ Peptone in a selective medium developed for isolating Leuconostoc spp. from food samples. BD Bacto™Peptone was used in a culture medium for two anaerobic, extremely thermophilic archaea, Thermococcus celer and Pyrococcus woesei, by Blamey et al.(3)
BD Bacto™ Peptone has also been utilized as a nitrogen source in mammalian cell culture media formulations. Taylor et al(4)used BD Bacto™ Peptone to supplement serum-free medium for several mammalian cell lines and reported the solubility of BD Bacto™ Peptone as very good at 10 g/100 mL of water.
Sakoda and Fukusho(5) also utilized BD Bacto™ Peptone in serum-free culture for maintaining porcine kidney epithelial cells. BD Bacto™ Peptone is also useful as a supplement in mammalian cell culture with serum.Researchers uncovered estrogenic activity associated with BD Bacto™ Peptone when observing that the estrone contained in BD Bacto™ Peptone was converted to estradiol by Saccharomyces cerevisiae after BD Bacto™ Peptone was added to the medium for culture of yeast. These findings suggest that adding estrogens to a medium containing BD Bacto™ Peptone for studies of estradiol production by yeast might confound results.(6,7) BD Bacto™ Peptone has also been used for the growth of the yeast Pichia for recombinant protein production,(8) and for xylitol production.(9)
References
1.Iwanaga, Yamamoto, Higa, Ichinose, Nakasone and Tanabe. 1986. Culture conditions for stimulating cholera toxin production by Vibrio cholerae O1 El Tor. Microbiol. Immunol. 30:1075-1083.
2. Benkerroum, Misbah, Sandine and Elaraki. 1993. Development and use of a selective medium for isolation of Leuconostoc spp. from vegetables and dairy products. Appl. Environ. Microbiol. 59:607-609.
3. Blamey, Chiong, Lopez and Smith. 1999. Optimization of the growth conditions of the extremely thermophilic microorganisms Thermococcus celer and Pyrococcus woesei. J. Microbiol. Methods 38:169-175.
4. Taylor, Dworkin, Pumper and Evans. 1972. Biological efficacy of several commercially available peptones for mammalian cells in culture. Exp. Cell Res. 74:275-279.
5. Sakoda and Fukusho. 1998. Establishment and characterization of a porcine kidney cell line, FS-L3, which forms unique multicellular domes in serum-free culture. In Vitro Cell. Dev. Biol. Anim. 34:53-57.
6. Feldman and Krishnan. 1995. Estrogens in unexpected places: possible implications for researchers and consumers. Environ. Health Perspect. 103 Suppl 7:129-133.
7. Miller, Bottema, Stathis, Tokes and Feldman. 1986. Unexpected presence of estrogens in culture medium supplements: subsequent metabolism by the yeast Saccharomyces cerevisiae. Endocrinology 119:1362-1369.
8.Wang, Yang, Lin, Tsai, Wu and Mao. 2002. Expression, characterization, and purification of recombinant porcine lactoferrin in Pichia pastoris. Protein Expression and Purification. 25:41-49.
9.Jin, Cruz and Jeffries. 2005. Xylitol production by a Pichia stipites D-xylulokinase mutant. Appl. Microbiol. Biotechnol. 68(1):42-45.
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