Culture et la numération des anaérobies, en particulier de Clostridium, à partir d'aliments et d'échantillons cliniques.

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Difco™ Reinforced Clostridial Medium

Intended Use
Reinforced Clostridial Medium is used for cultivating and enumerating clostridia, other anaerobes, and other species of bacteria from foods and clinical specimens.
Meets United States Pharmacopeia (USP), European Pharmacopoeia (EP) and Japanese Pharmacopoeia (JP) performance specifications, where applicable.

Summary and Explanation
Reinforced Clostridial Medium is a semisolid medium formulated by Hirsch and Grinstead. Their work demonstrated that the medium outperformed other media in supporting growth of clostridia from small inocula and produced higher viable cell counts. Barnes and Ingram used the medium to dilute vegetative cells of Clostridium perfringens. Barnes et al. used a solid (agar) version of the medium to enumerate clostridia in food.
Reinforced Clostridial Medium is a nonselective enrichment medium and grows various anaerobic and facultative bacteria when incubated anaerobically. This medium has been used to detect clostridia, bifidobacteria and other anaerobes in food products and fecal samples. Reinforced Clostridial Medium is listed in the USP as the recommended medium for the isolation of Clostridium sp. from nonsterile pharmaceutical products.

Principles of the Procedure
Reinforced Clostridial Medium contains peptone and beef extract as sources of carbon, nitrogen, vitamins and minerals. Yeast extract supplies B-complex vitamins which stimulate bacterial growth. Dextrose is the carbohydrate source. Sodium chloride maintains the osmotic balance. In low concentrations, soluble starch detoxifies metabolic by-products. Cysteine HCl is the reducing agent. Sodium acetate acts as a buffer. The small amount of agar makes the medium semisolid.

Formula
Difco™ Reinforced Clostridial Medium
=> Approximate Formula* Per Liter :
   - Peptone : 10.0 g
   - Beef Extract : 10.0 g
   - Yeast Extract : 3.0 g
   - Dextrose : 5.0 g
   - Sodium Chloride : 5.0 g
   - Soluble Starch : 1.0 g
   - Cysteine HCl : 0.5 g
   - Sodium Acetate : 3.0 g
   - Agar : 0.5 g
*Adjusted and/or supplemented as required to meet performance criteria.