Le Trypticase™ Soy Agar (TSA - Soybean-Casein Digest Agar ) est utilisé pour l'isolation et la culture des microorganismes fastidieux et non-fastidieux.

GranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.

Solubility : 40 g/l
pH : 7.1 < pH < 7.5 (40 g/l, H₂O, 25°C) (after autoclaving)
Bulk density : 620 kg/m3
Shipped in ambient
Storage temp.: Store at +15°C to +25°C.

Specifications :
      Appearance (clearness): clear
      Appearance (colour): yellowish brown
      pH-value (25 °C): 7.1 - 7.5
      Solidification behaviour (2 hrs., 45 °C): liquid
      Stability test (Colour and hemolysis): non-hemolytic
      
    Typical composition (g/litre): Pancreatic digest of casein 15.0; Papaic digest of soya bean 5.0; Sodium chloride 5.0; Agar-Agar 15.0.
      Groth promotion test in accordance with the harmonised method of EP, USP and JP.
      
    Inoculum on reference medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): 10 - 100
      Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003)): 10 - 100
      Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): 10 - 100
      Inoculum on reference medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)): 10 - 100
      Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)): 10 - 100
      Inoculum on reference medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): 10 - 100
      
      Recovery on test medium (Staphylococcus aureus ATCC 6538 (WDCM 00032)): ≥ 70 %
      Recovery on test medium (Bacillus subtilis ATCC 6633 (WDCM 00003)): ≥ 70 %
      Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 70 %
      Recovery on test medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026)): ≥ 70 %
      Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): ≥ 70 %
      Recovery on test medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): ≥ 50 %
      
    Incubation: 24 hrs.; 30-35°C; aerobic; C.albicans and Aspergillus brasiliensis up to 5 days
    
    Reference media: blood agar and SABOURAUD-Agar for C.albicans and Aspergillus brasiliensis
      
    The information above is current at this time of publication and is subject to change without notice (exept for customers holding a change control agreement with our company). The information/format can be altered at any time and in any way if internal company matters or Standard Regulations do say so.
      Growth promotion test in accordance with the current version of DIN EN ISO 11133.
      
      Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 70 %
      Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 70 %
      Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≥ 70 %
      Recovery on test medium (Listeria monocytogenes ATCC 13932 (WDCM 00021)): ≥ 70 %
      Recovery on test medium (Staphylococcus aureus ATCC 25923 (WDCM 00034)): ≥ 70 %
      
    Incubation: 24 ± 3 hours at 37 ± 1 °C
    Reference media: Tryptic Soy Agar
    A recovery rate of 70 % is equivalent to a productivity value of 0.7.
    The indicated colony counts result from the sum of a triple determination.