Application : Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Quantity : Formulated to contain 13.4 grams of powder per liter of medium.

Reconstitution : Supplement with 3.7 g/L sodium bicarbonate.

Dulbecco’s Modified Eagle’s Medium (DME)
Many modifications of Eagle’s Medium have been developed since the original formulation appeared in the literature. Among the most widely used of these modifications is Dulbecco’s Modified Eagle’s Medium (DME).
DME is a modification of Basal Medium Eagle (BME) that contains a 4-fold higher concentration of amino acids and vitamins, as well as additional supplementary components. The original DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. An alteration with 4,500 mg/L.glucose is optimal in cultivating certain cell types.

Inorganic Salts :
- CaCl₂ : 0.2 g/l,
- Fe(NO₃)₃ • 9H₂O : 0.0001 g/l,
- MgSO₄ : 0.09767 g/l,
- KCl : 0.4 g/l,
- NaHCO₃ : 3.7 g/l,
- NaCl : 6.4 g/l,
- NaH₂PO₄ : 0.109 g/l,

Amino Acids :
- L-Arginine • HCl : 0.084 g/l,
- L-Cysteine • 2HCl : 0.0626 g/l,
- L-Glutamine : 0.584 g/l,
- Glycine : 0.03 g/l,
- L-Histidine • HCl • H₂O : 0.042 g/l,
- L-Isoleucine : 0.105 g/l,
- L-Leucine : 0.105 g/l,
- L-Lysine • HCl : 0.146 g/l,
- L-Methionine : 0.03 g/l,
- L-Phenylalanine : 0.066 g/l,
- L-Serine : 0.042 g/l,
- L-Threonine : 0.095 g/l,
- L-Tryptophan : 0.016 g/l,
- L-Tyrosine • 2Na • 2H₂O : 0.10379 g/l,
- L-Valine : 0.094 g/l,

Vitamins :
- Choline Chloride : 0.004 g/l,
- Folic Acid : 0.004 g/l,
- myo-Inositol : 0.0072 g/l,
- Niacinamide : 0.004 g/l,
- D-Pantothenic Acid • ½Ca : 0.004 g/l,
- Pyridoxal • HCl : 0.004 g/l,
- Pyridoxine • HCl : 0.00404 g/l,
- Riboflavin : 0.0004 g/l,
- Thiamine • HCl : 0.004 g/l,

Other :
- D-Glucose : 4.5 g/l,
- Phenol Red • Na : 0.0159 g/l,

ADD :
- L-Glutamine : 0.584 g/l,
- NaHCO₃ : 3.7 g/l.