Culture et dénombrement des Lactobacillus dans les produits laitiers et autres produits alimentaires.

Intended Use
Lactobacilli MRS Agar and Lactobacilli MRS Broth are recommended for use in the isolation, enumeration and cultivation of Lactobacillus species.

Summary and Explanation
Lactobacilli MRS Agar and Lactobacilli MRS Broth are based on the formulations of deMan, Rogosa and Sharpe. These media were shown by the authors to support luxuriant growth of all lactobacilli from oral, fecal, dairy and other sources.

Principles of the Procedure
Lactobacilli MRS Agar and Lactobacilli MRS Broth contain peptone and dextrose. These ingredients supply nitrogen, carbon and other elements necessary for growth. Polysorbate 80, acetate, magnesium and manganese provide growth factors for culturing a variety of lactobacilli. The above ingredients may inhibit the growth of some organisms other than lactobacilli.

Difco™ Lactobacilli MRS Broth
Dehydrated Appearance: Tan, homogeneous, appears moist.
Solution: 5.5% solution, soluble in purified water upon boiling. Solution is medium amber, clear to very slightly opalescent.
Prepared Appearance: Medium amber, clear to very slightly opalescent.
Reaction of 5.5%
Solution at 25°C: pH 6.5 ± 0.2