Solubility : 40 mg/ml
pH : 7
Molecular weight : ~66 kDa
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
 

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Application
• Bovine Serum Albumin has been used as a supplement in the medium prepared for culturing of oligodendrocytes.
• It has been used for the dilution of primary antibody solution in immunohistochemistry procedure.
• It has been used as a standard in the Bradford method of protein quantification.
• It has been used in the blocking and permeabilization buffer during immunofluorescence studies.

Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

General description
Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids. It is a water-soluble protein with a molecular weight of 66.4 kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

Storage temp.: 2-8°C.

Poids	1,7 Kg
Volume	0,008 m³   (8000 cm³)
Largeur	0,2 m   (20 cm)
Longueur	0,2 m   (20 cm)
Hauteur	0,2 m   (20 cm)

Les dimensions, le poids et le volume sont bruts (emballage inclus)